This assay has been validated for serum, EDTA and heparin plasma, urine samples and for tissue culture samples.
#Detectx free software
Software for converting raw relative optical density readings from the plate reader and carrying out four parameter logistic curve (4PLC) fitting. Repeater pipet with disposable tips capable of dispensing 25 μL, 50 μL and 100 μL.Ĭolorimetric 96 well microplate reader capable of reading optical density at 450 nm. Stop Solution A 1M solution of hydrochloric acid. Wash Buffer Concentrate A 20X concentrate that should be diluted with deionized or distilled water. 28 mL or 55 mLĭissociation Reagent is to be used only with Serum and plasma samples. 3 mL or 13 mLĪssay Buffer Concentrate A 5X concentrate that must be diluted with deionized or distilled water. 3 mL or 13 mLĭetectX® Corticosterone Conjugate A corticosterone-peroxidase conjugate in a special stabilizing solution. 125 or 625 μLĭetectX® Corticosterone Antibody A sheep polyclonal antibody specific for corticosterone. 1 or 5 EachĬorticosterone Standard Corticosterone at 100,000 pg/mL in a special stabilizing solution. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.Ĭoated Clear 96 Well plates A clear plastic microtiter plate(s) coated with donkey anti-sheep IgG. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. After a short incubation, the reaction is read at 450nm. The substrate reacts with the bound corticosterone-peroxidase conjugate.
After an hour incubation the plate is washed and substrate is added.
The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone. A corticosterone-peroxidase conjugate is added to the wells. Diluted samples are pipetted into a clear microtiter plate coated with an antibody. The kit has been developed for extracted fecal samples and has been validated with multiple animal species. The Corticosterone Enzyme Immunoassay kit measures corticosterone present in tissue culture, serum, plasma and fecal samples. Sample Types validated: Dried Fecal Extracts, Serum, EDTA and Heparin Plasma and Tissue Culture Media Serum, Plasma (EDTA), Plasma (heparin), Urine, Fecal, Tissue Culture Medium The DetectX® Corticosterone Immunoassay kit is designed to quantitatively measure Corticosterone present in extracted dried fecal samples, serum, plasma and tissue culture media samples. Annexin V-FITC Apoptosis Detection Kits.
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